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Related Experiment Videos

Particle-associated microorganisms in stormwater runoff.

Michael Borst1, Ariamalar Selvakumar

  • 1Urban Watershed Management Branch, United States Environmental Protection Agency (MS-104), Edison, NJ 08837, USA.

Water Research
|December 6, 2002
PubMed
Summary

Blending and chemical treatment significantly altered microbial concentrations in stormwater runoff. These methods impact the accuracy of microorganism analysis, affecting our understanding of water quality and microbial ecology.

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Area of Science:

  • Environmental Microbiology
  • Water Quality Analysis
  • Microbial Ecology

Background:

  • Accurate enumeration of microorganisms in stormwater runoff is crucial for assessing water quality and ecological impact.
  • The role of clumped or particle-associated microorganisms in stormwater analysis requires further investigation.
  • Pre-analytical sample processing methods, such as blending and chemical addition, can influence microbial concentration measurements.

Purpose of the Study:

  • To investigate the effects of sample blending and chemical addition on microorganism concentrations in stormwater runoff.
  • To determine if clumped or particle-associated organisms significantly influence microbial enumeration in stormwater.
  • To assess the impact of pre-analytical treatments on the accuracy of standard membrane filtration methods for stormwater analysis.

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Main Methods:

  • Stormwater runoff samples were collected from a single summer storm.
  • Samples were subjected to blending (22,000 rpm) and/or chemical treatment (Camper's solution).
  • Microorganism concentrations (total coliform, fecal coliform, Escherichia coli, fecal streptococci) were enumerated using the standard membrane filtration method.

Main Results:

  • Blending significantly increased measured concentrations for most tested organisms (except Escherichia coli).
  • Camper's solution generally decreased organism concentrations, with notable exceptions for fecal streptococci.
  • A statistically significant interaction was observed between blending and Camper's solution effects for most organisms, excluding total coliform. Blending did not significantly alter mean particle size.

Conclusions:

  • Pre-analytical sample processing methods (blending and chemical treatment) significantly affect microorganism concentration measurements in stormwater runoff.
  • The study found no correlation between increased total coliform, fecal coliform, and fecal streptococcus concentrations and mean particle size.
  • These findings highlight the importance of standardizing pre-analytical procedures to ensure reliable microbial analysis of stormwater.