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Related Experiment Videos

Telomerase reverse transcriptase subunit immunoreactivity: a marker for high-grade prostate carcinoma.

Kenneth A Iczkowski1, Cooley G Pantazis, Douglas H McGregor

  • 1Department of Pathology, University of Florida, Gainesville, Florida, USA. iczkoka@pathology.ufl.edu

Cancer
|December 6, 2002
PubMed
Summary

Telomerase reverse transcriptase (TERT) nuclear reactivity increases with prostate cancer grade. High-grade tumors show consistent TERT reactivity, suggesting they may be most responsive to telomerase-targeted therapies.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Telomerase maintains telomeric DNA and is found in most prostate carcinomas.
  • Previous detection methods include PCR and in situ hybridization.
  • Immunohistochemical data for telomerase in prostate cancer were previously unavailable.

Purpose of the Study:

  • To investigate telomerase reverse transcriptase (TERT) expression in prostate cancer using immunohistochemistry.
  • To correlate TERT expression with tumor grade, stage, and biochemical failure.
  • To evaluate TERT as a potential marker in prostate cancer progression.

Main Methods:

  • 62 prostatectomy tissue sections were immunostained for TERT.
  • Staining was evaluated by two observers and correlated with clinicopathological data.

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  • Tumors were categorized by Gleason score (3-10).

    • Main Results:

    • TERT nuclear reactivity increased significantly with tumor grade (P < 0.0001).
    • 100% of high-grade tumors (Gleason 8-10) showed TERT reactivity.
    • TERT reactivity was not correlated with pathologic stage or margin status.
    • TERT reactivity was observed in basal cells of nonneoplastic glands and HGPIN, and in secretory cells of HGPIN and tumors.

    Conclusions:

    • Nuclear anti-TERT reactivity serves as a basal cell marker in nonneoplastic prostate glands.
    • TERT expression is acquired by secretory cells during tumorigenesis.
    • Consistent TERT reactivity is primarily seen in high-grade prostate carcinomas (Gleason primary pattern ≥4).
    • High-grade tumors exhibit maximal telomerase activation, indicating potential responsiveness to antitelomerase therapies.