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Updated: Jul 28, 2026
09:16
Eukaryotic Polyribosome Profile Analysis
Published on: June 15, 2010
Comprehensive sequence analysis of translation termination sites in various eukaryotes
1Institute for Advanced Biosciences, Keio University, 5322 Endo, Fujisawa, Japan.
Gene
|December 7, 2002
Summary
Sequences around stop codons influence translation termination efficiency. This study found a consensus sequence at position +1 in mammals, correlating with translation efficiency and codon bias.
Area of Science:
- Molecular Biology
- Genomics
- Bioinformatics
Background:
- Translation termination is crucial for protein synthesis.
- Sequences flanking stop codons, not just the codons themselves, impact termination efficiency.
Purpose of the Study:
- To investigate the relationship between sequence patterns around stop codons and translation termination efficiency.
- To analyze the correlation between the strength of consensus sequences and codon bias.
Main Methods:
- Sequence profile analysis of translation termination sites using mouse cDNA and eukaryotic UniGene datasets.
- Correlation analysis between consensus strength, information content, and Codon Adaptation Index (CAI).
Main Results:
- A strong consensus sequence (G and A at position +1) was identified in mouse cDNA and other mammals.
- Mouse cDNA showed a positive correlation between CAI and information content at position +1.
- Strong consensus sequences generally correlated positively with CAI in other eukaryotes.
Conclusions:
- Consensus sequences around stop codons play a role in regulating translation efficiency.
- Codon bias and gene expression levels are linked to these sequence patterns.
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