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Preparation and storage of control slides for fetal hemoglobin determination by acid elution

J K Barr, J A Shafer

    The American Journal of Medical Technology
    |January 1, 1976
    PubMed

    To simplify the reading of acid eluted blood smears for fetal hemoglobin, positive and negative controls are placed side by side on a single slide. Red blood cells having high Hb F content are stained a bright pink in contrast to cells devoid of Hb F which appear as ghost cells with a faint pink outline. A method for preparing, fixing, and short-term storing of control slides with their subsequent room temperature acid elution and staining is given.

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