Interleukin-12 inhibits apoptosis in chronic lymphatic leukemia (CLL) B cells
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Updated: Sep 28, 2026

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
Published on: October 23, 2019
S Schuhknecht1, S Duensing, I Dallmann
1Medizinische Hochschule Hannover, Hämatologie und Onkologie, Hannover, Germany.
Abstract:
The purpose of the present study was to investigate the effect of interleukin-12 on apoptosis of chronic lymphatic leukemia (CLL) B cells. Apoptotic indices were determined in highly purified CD5(+) B lymphocytes isolated from peripheral blood of seven patients with histologically confirmed CLL. Interleukin-4 as a known inhibitor of apoptosis was used as control. Quantitative analysis of apoptosis was determined by cell death detection ELISA. Our findings indicate that interleukin-12 inhibits ex vivo apoptosis in a large proportion of B-CLL patients and may be closely involved in the pathogenesis of disease. Therefore, our results may help identify potential new therapeutic targets in this malignancy.
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