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Monoclonal antibody against free beta-subunit of human chorionic gonadotropin
Ileana B Baluja Conde1, Adys I Brito Moreno, Carmen Acosta Bas
1Laboratorio de Anticuerpos Monoclonales, Centro de Inmunoensayo, Apartado 6653, Ave 25 y 134, Cubanacán, Playa, Ciudad Habana, Cuba. iqmonoclol@cie.sld.cu
Abstract:
Spleen cells from BALB/c mice immunized with free native human chorionic gonadotropin hormone beta-subunit (beta hCG) were fused with mouse myeloma cells (P3/X63-Ag8) and one hybridoma secreting monoclonal antibodies (MAbs), was obtained. This hybridoma specifically recognizes beta hCG and does not cross-react with other human glycoprotein hormones, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The MAb was of the IgG(1) subclass and ascitic fluid from this hybridoma was purified by affinity chromatography on Protein A-Sepharose CL-4B column to isolate the IgG(1) active fraction. The affinity constant of this MAb was 1.5 x 10(10)M(-1).