Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fundamental cellular processes do not require vertebrate-specific sequences within the TATA-binding protein.

Edward E Schmidt1, Alla A Bondareva, Jay R Radke

  • 1Department of Veterinary Molecular Biology, Marsh Laboratories, Montana State University, Bozeman, Montana 59717, USA. eschmidt@montana.edu

The Journal of Biological Chemistry
|December 10, 2002
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prime editing-mediated microhomology enables efficient replacement of large DNA.

Nucleic acids research·2026
Same author

Cystine C-S bond cleavage fuels cysteine production under disulfide reductase deficiency.

Nature chemical biology·2026
Same author

Microglia respond to and induce anxiety and grooming in mice using calcium signaling.

Molecular psychiatry·2026
Same author

Primary bovine embryonic fibroblasts demonstrate variable fitness following infection with highly pathogenic avian influenza H5N1 strains and are susceptible to a recently circulating human 2009 pandemic lineage H1N1 strain.

Microbiology spectrum·2026
Same author

Basic enables selection-free efficient knockin of large DNA in primary human T cells.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Defective Hoxb8 microglia are causative for both chronic anxiety and pathological overgrooming in mice.

Molecular psychiatry·2025

Vertebrate TATA-binding protein (TBP) N-terminal deletions cause midgestation lethality in mice. However, basic cellular functions remain unaffected in fibroblasts lacking these vertebrate-specific sequences.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • TATA-binding protein (TBP) is crucial for transcription initiation across eukaryotes.
  • Vertebrate TBP possesses a unique N-terminal region absent in other phyla.
  • The function of this vertebrate-specific TBP region is largely unknown.

Purpose of the Study:

  • To investigate the in vivo function of the vertebrate-specific N-terminal region of TBP.
  • To determine if deletion of this region impacts general cellular processes.
  • To elucidate the cause of midgestation lethality in mice with TBP N-terminal deletions.

Main Methods:

  • Generated mice with a TBP allele lacking 111 N-terminal amino acid residues (tbp(Delta N)).
  • Established primary fibroblast cultures from wild-type, heterozygous, and homozygous mutant fetuses.

Related Experiment Videos

  • Assessed cell proliferation, gene expression (DHFR, cdc25B, U6 RNA, housekeeping genes), transcription initiation fidelity (pol II, pol III), and mRNA splicing.

    • Main Results:

    • Homozygous tbp(Delta N/Delta N) fetuses exhibited midgestation lethality.
    • Fibroblast cultures showed no genotype-dependent differences in proliferation or expression of proliferation markers.
    • No significant impact was observed on transcription initiation fidelity, U6 RNA levels, mRNA splicing, or global gene expression.

    Conclusions:

    • The vertebrate-specific N-terminal sequences of TBP are dispensable for fundamental eukaryotic cell functions in vitro.
    • The observed midgestation lethality is likely due to functions not represented in primary fibroblasts or compensated by redundant pathways.
    • Further research is needed to identify the specific roles and compensatory mechanisms related to the TBP N-terminal domain during development.