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[An experimental study on c-myc gene mRNA expression of endothelial cell induced by bFGF]
Zhigang Zhou1, Qinglian He, Zihao Lin
1Department of Plastic Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Objective:
To investigate the molecular biological mechanism of endothelial cell proliferation induced by bFGF.
Methods:
Cultured rat myocardial microvascular endothelial cells were stimulated with bFGF of various concentrations. By northern blot analysis, the levels of c-myc mRNA expression were detected.
Results:
The expression of c-myc mRNA in the bFGF-treated groups increased (P < 0.05) with a dose- and stimulating time-dependent manner. The c-myc mRNA expression reached to a peak level at 2 hours.
Conclusions:
C-myc expression may be an important component in controlling the transit of cells through the cell cycle.