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Sperm evaluation in cryopreserved bovine semen recovered by two selection methods
G M Trentalance1, N B Beorlegui
1Biological Chemistry Area, School of Veterinary Sciences, University of Buenos Aires, Buenos Aires, Argentina.
Andrologia
|December 11, 2002
Summary
Two methods for selecting sperm from frozen-thawed bull semen, Percoll gradient centrifugation and Sephadex ion-exchange filtration, significantly improved sperm motility and acrosome integrity. Both techniques effectively selected viable sperm, though with a reduced recovery rate.
Area of Science:
- Veterinary Reproduction
- Sperm Biology
- Cryopreservation Technology
Background:
- Semen manipulation techniques enhance sperm quality for assisted reproduction.
- Limited comparative data exists for frozen-thawed bovine semen selection methods.
Purpose of the Study:
- To compare Percoll gradient centrifugation and Sephadex ion-exchange filtration for selecting motile, morphologically normal spermatozoa from cryopreserved bovine semen.
- To assess sperm damage induced by these selection methods.
Main Methods:
- Comparison of discontinuous Percoll gradient centrifugation versus Sephadex G-15-120 ion-exchange column filtration.
- Evaluation of sperm concentration, motility, acrosome integrity, superoxide dismutase activity, and lipid peroxidation in selected and control samples.
Main Results:
- Both Sephadex filtration (88.87%) and Percoll centrifugation (83.00%) significantly increased sperm motility compared to controls (60.14%).
- Both methods significantly enhanced acrosome integrity and superoxide dismutase activity.
- A significant reduction in recovered spermatozoa count was observed for both selection methods.
Conclusions:
- Percoll gradient centrifugation and Sephadex ion-exchange filtration are effective in selecting viable spermatozoa from cryopreserved bovine semen.
- These methods improve key sperm quality parameters, despite a lower yield of recovered sperm.