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Serological differences in three Blastomyces dermatitidis strains.

R C Axtell1, G M Scalarone

  • 1Department of Biological Sciences, Idaho State University, Pocatello, Idaho 83209, USA.

Mycoses
|December 11, 2002
PubMed
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Three Blastomyces dermatitidis yeast lysate antigens were tested for antibody detection. Two antigens showed high reactivity with blastomycosis-infected dog sera, while one Zaire isolate antigen displayed distinct serological differences, potentially indicating a new serotype.

Area of Science:

  • Mycology
  • Immunology
  • Infectious Diseases

Background:

  • Blastomycosis is a fungal infection caused by Blastomyces dermatitidis.
  • Accurate serological diagnostic tools are crucial for identifying blastomycosis.
  • Antigenic variability within B. dermatitidis may impact diagnostic test performance.

Purpose of the Study:

  • To evaluate the reactivity of three Blastomyces dermatitidis yeast phase lysate antigens against human, dog, and rabbit sera.
  • To identify potential serological differences among B. dermatitidis isolates.
  • To assess the diagnostic utility of these antigens for blastomycosis.

Main Methods:

  • Preparation of yeast phase lysate antigens from three B. dermatitidis isolates (T-58, 48089, ERC-2).
  • Assay of antigen reactivity using sera from blastomycosis-diagnosed dogs, humans exposed to B. dermatitidis, and immunized rabbits.

Related Experiment Videos

  • Analysis of antibody detection capabilities of each lysate antigen.

    • Main Results:

    • T-58 and ERC-2 lysate antigens showed high reactivity with sera from dogs with blastomycosis.
    • The 48089 (Zaire) lysate antigen exhibited low reactivity with dog blastomycosis sera but high reactivity with homologous rabbit immune serum.
    • All three lysate antigens successfully detected antibodies in humans exposed to B. dermatitidis.

    Conclusions:

    • Serological differences exist among the tested Blastomyces dermatitidis lysate antigens.
    • The 48089 Zaire isolate antigen displays unique reactivity patterns, suggesting it may represent a distinct serotype (serotype 2).
    • Further investigation into B. dermatitidis serotypes is warranted for improved diagnostics.