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Related Experiment Videos

Novel fish hypothalamic neuropeptide.

Kaori Sawada1, Kazuyoshi Ukena, Honoo Satake

  • 1Laboratory of Brain Science, Faculty of Integrated Arts and Sciences, Hiroshima University, Higashi-Hiroshima, Japan.

European Journal of Biochemistry
|December 11, 2002
PubMed
Summary
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Researchers discovered a novel neuropeptide in goldfish brains, similar to those found in birds and amphibians. This peptide, containing the LPLRF-NH2 motif, is expressed in the hypothalamus and may regulate pituitary hormone release.

Area of Science:

  • Neuroendocrinology
  • Comparative Endocrinology
  • Molecular Biology

Background:

  • Novel hypothalamic neuropeptides inhibiting gonadotropin release and stimulating growth hormone release were previously identified in avian and amphibian species.
  • These neuropeptides share a conserved C-terminal LPLRF-NH2 motif, suggesting a conserved function across vertebrates.

Purpose of the Study:

  • To investigate the conservation of novel hypothalamic neuropeptides in vertebrates.
  • To characterize a novel neuropeptide and its gene expression in the goldfish (Carassius auratus) brain.

Main Methods:

  • 3' and 5' rapid amplification of cDNA ends (RACE) to identify the cDNA.
  • Mass spectrometry to identify the endogenous ligand.
  • Southern blotting and reverse-transcriptase-mediated PCR to analyze gene expression.

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  • In situ hybridization to determine mRNA localization.

    • Main Results:

    • A cDNA encoding a precursor for a novel neuropeptide with a C-terminal -LPXRF motif was identified in goldfish.
    • Mass spectrometry confirmed a tridecapeptide (SGTGLSATLPQRF-NH2) as the endogenous ligand.
    • The goldfish peptide gene is specifically expressed in the diencephalon, with mRNA localized to the nucleus posterioris periventricularis.
    • Immunoreactive cell bodies and fibers were found in the hypothalamus, nervus terminalis, and pituitary.

    Conclusions:

    • The goldfish hypothalamus expresses a novel neuropeptide containing the C-terminal -LPQRF-NH2 sequence.
    • This novel neuropeptide may have multiple regulatory functions, potentially including the regulation of pituitary hormone release.