Related Experiment Videos
[The functional and phenotypic analysis of T lymphocytes under stimulation of THANK]
Dong Wu1, Feng Shen, Mengchao Wu
1Eastern Hepatobiliary Hospital, Shanghai, 200438 China.
Zhonghua Yi Xue Za Zhi
|December 12, 2002
Objective:
To study the function of THANK in T lymphocyte immune regulation.
Methods:
On the basis of THANK gene cloning and expression, isolated T and B lymphocytes were stimulated by purified THANK protein and its function in immune regulation was investigated by (3)HTdR incorperation and flow cytometric analysis.
Results:
In addition to the costimulation on B cell growth, THANK promoted CD3-mediated T cells proliferation, activated resting peripheral blood T lymphocytes and induced apoptosis and the phenotypic change of activated T lymphocytes.
Conclusion:
THANK is involved in the modulation of T cells phenotype and function.