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[The activity of M-phase promoting factor in oral normal tissue and tumor]
Yi Liu1, Yulou Tian, Aiming Yu
1Department of Orthodontics, School of Stomatology, China Medical University, Shenyang 110002, China.
Objective:
To investigate the content and activity of M-phase promoting factor (MPF) in pleomorphic adenoma, mucoepidermoid carcinoma, buccal carcinoma and normal tissue, in order to evaluate the role of MPF in the development of tumor and the relationship between MPF and malignant degree.
Methods:
The content and activity of MPF were assessed by immunobloting and Gollicano method.
Results:
The cdc2 and cyclinB (two subunits of MPF) were found both in normal and tumor tissues, and their content in tumor was higher than normal tissues. Buccal carcinoma was 64% higher than normal tissues. The activity of MPF in carcinoma was higher than normal tissue and had positive relation with the malignant extent.
Conclusions:
The content and activity of MPF in tumor are higher than normal tissue. PKC can activate MPF. These results show PKC may promote tumor proliferation by activating MPF and also, the activity of MPF has some relation with malignant extent.
Insights
M-phase promoting factor (MPF) content and activity are elevated in tumors compared to normal tissues. Protein kinase C (PKC) activation of MPF may drive tumor proliferation and correlate with malignancy.
Area of Science:
- Cell cycle regulation
- Cancer biology
- Biochemistry
Context:
- M-phase promoting factor (MPF), a key regulator of cell division, comprises cdc2 and cyclinB subunits.
- Aberrant cell cycle control is a hallmark of cancer development.
- Understanding MPF's role in oral tumors is crucial for targeted therapies.
Purpose:
- To investigate MPF content and activity in pleomorphic adenoma, mucoepidermoid carcinoma, buccal carcinoma, and normal tissues.
- To evaluate MPF's role in tumor development and its relationship with malignant degree.
- To explore the potential involvement of Protein Kinase C (PKC) in MPF regulation within oral cancers.
Summary:
- Immunoblotting and Gollicano methods revealed higher MPF content and activity in tumor tissues than normal tissues.
- Buccal carcinoma showed a 64% increase in MPF compared to normal tissues.
- MPF activity positively correlated with the malignant extent of oral tumors.
Impact:
- Elevated MPF content and activity in tumors suggest its involvement in proliferation.
- PKC activation of MPF presents a potential mechanism for promoting tumor growth.
- MPF activity serves as a potential biomarker for oral cancer malignancy.