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Related Experiment Videos

Light elements quantification in stimulated cells cryosections studied by electron probe microanalysis.

Patrice Laquerriere1, Jean Michel, Gerard Balossier

  • 1Laboratoire de Micoscopie Electronique, EA 2068, IFR 53, UFR Sciences, 21 rue Clément Ader, BP 138, 51685 Reims Cedex 2, France. laquerrp@ors.od.nih.gov

Micron (Oxford, England : 1993)
|December 12, 2002
PubMed
Summary

Stimulating microglial cells with gamma-interferon and lipopolysaccharide increases intracellular oxygen but not nitrogen. This suggests external oxygen use, not solely nitric oxide (NO) production, in cellular responses.

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Area of Science:

  • Cell Biology
  • Microscopy Techniques
  • Biochemistry

Background:

  • Intracellular light element quantification (carbon, nitrogen, oxygen) aids understanding biological mechanisms like nitric oxide (NO) generation in apoptosis and inflammation.
  • Electron energy loss spectroscopy (EELS) and energy-dispersive X-ray spectroscopy (EDS) are key techniques for detecting light elements in biological samples.

Purpose of the Study:

  • To investigate changes in intracellular oxygen and nitrogen concentrations in BV-2 murine microglial cells upon stimulation.
  • To compare the efficacy of EELS and EDS for quantifying light elements in stimulated microglial cells.

Main Methods:

  • Utilized scanning transmission electron microscopy (STEM) combined with EELS.
  • Employed EDS with advanced X-ray detectors.

Related Experiment Videos

  • Stimulated BV-2 murine microglial cells with gamma-interferon and lipopolysaccharide.

    • Main Results:

    • Observed a significant increase in intracellular oxygen concentration following stimulation.
    • Detected no significant change in intracellular nitrogen concentration.
    • Quantified NO production, finding it 1000 times lower than the observed oxygen increase.

    Conclusions:

    • Stimulation of BV-2 cells involves the uptake of exogenous molecular oxygen.
    • The observed oxygen increase is not solely attributable to nitric oxide production.
    • EELS and EDS are effective methods for analyzing elemental changes in stimulated microglial cells.