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[Cloning of human brain-derived neurotrophin-6 gene and its expression in procaryotic cell]
Chengwu Zhang1, Qingsong Cai, Xu Zhuang
1Department of Physiology, School of Pre-clinical and Forensic Medicine, Sichuan University, Chengdu, Sichuan, 610041 P. R. China.
Objective:
To clone human brain-derived neurotrophin-6(NT-6) gene and to observe its expression in the procaryotic cell.
Methods:
Total RNA was extracted from aborted antenatal cerebral cortex, and cDNA fragment of NT-6 was amplified through reverse transcript-polymerase chain reaction. After being incised and recovered, the NT-6 gene was cloned into pBK-CMV plasmid to construct a NT-6 gene expression vector. Expression of NT-6 gene in Escherichia coli was studied after being induced by isopropyl beta-D-thiogalactoside(IPTG).
Results:
The NT-6 gene expression vector was constructed and Escherichia coli with recombinant vector expressed specific protein after induction by IPTG.
Conclusion:
The cloning of human brain-derived NT-6 gene provides a basis for further studying the structure, function and clinical application of NT-6.
