Related Experiment Video

Updated: Aug 26, 2026

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation
10:35

Assessment of Antibody-based Drugs Effects on Murine Bone Marrow and Peritoneal Macrophage Activation

Published on: December 26, 2017

[The comparative effects of actinomycin D, imuren and hydrocortisone on interferon and antibody formation in mice]

V D Solov'ev, A M Sorokin

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |January 1, 1976
    PubMed

    Insights

    Actinomycin-D and hydrocortisone significantly reduced interferon production. Actinomycin-D also abolished antibody production, suggesting distinct cellular mechanisms in antiviral immunity.

    Area of Science:

    • Immunology
    • Virology
    • Cell Biology

    Context:

    • Investigating the differential effects of immunosuppressive agents on antiviral responses.
    • Understanding the cellular basis of interferon and antibody production.

    Purpose:

    • To compare the impact of actinomycin-D and hydrocortisone on viral interferon and antibody production.
    • To elucidate the cellular heterogeneity involved in interferon synthesis.

    Summary:

    • Actinomycin-D (2.5 mg/kg) reduced interferon 16-fold and abolished antibody production.
    • Hydrocortisone (100 mg/kg) reduced interferon 16-fold but did not affect antibody production.
    • These findings suggest distinct cell populations are responsible for interferon production.

    Impact:

    • Provides insights into the differential regulation of innate and adaptive antiviral immunity.
    • Highlights the distinct mechanisms of action for actinomycin-D and hydrocortisone in immune suppression.
    • Supports the hypothesis of cellular heterogeneity in the interferon response.

    More Related Videos

    Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms11:36

    Induction of Drug-Induced, Autoimmune Hepatitis in BALB/c Mice for the Study of Its Pathogenic Mechanisms

    Published on: May 29, 2020

    Murine Model of Epicutaneously-Induced Immunomodulation09:07

    Murine Model of Epicutaneously-Induced Immunomodulation

    Published on: June 24, 2025

    Related Concept Videos

    Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents01:29

    Drugs for Treatment of Crohn's Disease in IBD Using Immunomodulatory Agents

    Crohn's disease is an inflammatory bowel disorder marked by chronic inflammation of the GI tract. Various treatment strategies for Crohn's disease are employed, such as immunomodulatory agents, glucocorticoids, and biologics or anti-TNF therapy. Azathioprine (Imuran), a commonly used immunomodulatory drug for Crohn's disease, is converted in the body to mercaptopurine, which inhibits purine biosynthesis and cell proliferation. Both are utilized in severe cases of Inflammatory Bowel Disease...
    Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF01:24

    Drugs for Treatment of Crohn's Disease in IBD Using Biologic Agents: Anti-TNF

    Tumor Necrosis Factor (TNF), a proinflammatory cytokine, contributes significantly to the inflammation seen in Crohn's disease. It exists as soluble TNF and membrane-bound TNF, with actions mediated through TNF receptors (TNFR). TNFR activation leads to the release of proinflammatory cytokines, T-cell activation, collagen production, and leukocyte migration, all contributing to inflammation in Crohn's disease. Anti-TNF monoclonal antibodies, namely infliximab (Remicade), adalimumab (Humira),...
    Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids01:21

    Drugs for Treatment of Crohn's Disease in IBD Using Glucocorticoids

    Glucocorticoids, a class of anti-inflammatory drugs, are pivotal in treating moderate to severe Crohn's disease by inducing remission. They exhibit their anti-inflammatory action by inhibiting the production of inflammatory cytokines such as tumor necrosis factor (TNF)-α, interleukin (IL)-1, and chemokines like IL-8. In addition, they reduce the expression of inflammatory cell adhesion molecules and inhibit gene transcription of nitric oxide synthase, phospholipase A2, cyclooxygenase-2 (COX-2),...