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Published on: August 18, 2015
Kupffer cell phagocytosis blockade decreases morbidity in endotoxemic rats with obstructive jaundice
1Department of Surgery, University of Szeged, Hungary. lg@surg.szote.u-szeged.hu
Objective And Design:
The consequences of Kupffer cell phagocytosis blockade were studied in endotoxemic rats with obstructive jaundice.
Material Or Subjects:
159 male Wistar rats.
Treatment:
Obstructive jaundice was induced by bile duct ligation (BDL). Gadolinium chloride (1 mg/100 g iv) was given 6 days after BDL to inhibit Kupffer cell activity and the animals were challenged with 1 microg/g endotoxin 24 h later.
Methods:
Endotoxin sensitivity, tumor necrosis factor-alpha and interleukin-6 production were studied, liver and lung injury were assessed by neutrophil infiltration assay, tissue adenosine triphosphate, aspartate aminotransferase, alanine aminotransferase level determinations and histology, respectively. For statistics non-parametric methods were used.
Results:
BDL sensitized the animals to endotoxin, increased endotoxin-induced tumor necrosis factor-alpha and interleukin-6 production and reduced ATP contents of the liver and the lung. Kupffer cell blockade significantly increased the resistance against endotoxin, diminished the inflammatory cytokine release and reduced endotoxin-induced tissue injury in BDL animals.
Conclusion:
Attenuation of Kupffer cell function decreases endotoxin-induced lethality and morbidity in obstructive jaundice.
