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Updated: Aug 13, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Sexual hormone and sperm cytological test in patients with aspermia]
Qu-Tao Zhang1, Jian-Feng Chang, Yi-Feng Ge
1Laboratory of Reproduction & Genetics, Nanjing General Hospital of Nanjing Command, PLA, Nanjing, Jiangsu 210002, China.
Objectives:
To test the serum sexual hormone and sperm cytology in aspermia patients.
Methods:
Radioimmunoassay (RIA) and Wright-Giemsa stain were used to detect serum sexual hormone and sperm cytology in 45 aspermia patients.
Results:
Among the 45 patients, spermatogenetic cells were detected in 16 patients(35.6%). The level of serum testerone were decreased in 16 patients, and FSH, LH were increased in 13 cases as well as that of PRL in 4 cases.
Conclusions:
These results suggested that the testis function was demaged, the ratio of T/LH could further reflect the function of Leydig cells, the detection of PRL were significant only in diagnosis of aspermia induced by hypermia. It is an important index in identifying obstructive and non-obstructive aspermia and has important significance in estimating the extention of testis demage and commanding the clinical treatment of serum hormone detection and sperm cell analysis.
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