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[Preliminary study on hens immunized with recombinant human testis prostaglandin D synthase DNA]
Jin-Chun Lu1, Yu-Feng Huang, Xi-Ran Zhang
1Clinical Laboratory, Nanjing Hospital of the Chinese Armed Police Force, Nanjing, Jiangsu 210024, China.
Objectives:
In order to identify whether the hens immunized with recombinant human testis prostaglandin D synthase (rhtL-PGDS) DNA can produce anti-L-PGDS antibody.
Methods:
The serum were got from the hens immunized with recombinant plasmid pGEX-2T/htL-PGDS DNA (100 micrograms) every 2 weeks for 2 times. The exist of anti-L-PGDS antibody and its titer were tested with agarose dual immunodiffusion and ELISA with rhtL-PGDS as antigen.
Results:
The serum anti-L-PGDS antibody in hen immunized with pGEX-2T/htL-PGDS DNA were confirmed and its titer tested by ELISA was 1:2,048.
Conclusions:
It is feasible to produce anti-L-PGDS antibody by immunizing hens with recombinant pGEX-2T/htL-PGES DNA.