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Updated: Sep 28, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
[Preliminary investigation of relationship between sperm apoptosis and male infertility]
Guang-Rong Wang1, Zeng-Di Zhou, Zheng-Min Ge
1Family Planning Research Institute of Yunnan, Kunming, Yunan 650021, China.
Objectives:
To evaluate the relationship between sperm apoptosis and male infertility.
Methods:
Percentage of apoptotic sperm (PAS) in spermatozoa of fertile and infertile men were tested by flow cytometry (FCM).
Results:
Sperm apoptosis had happened in all different people. PAS in fertile and infertile group was (4.28 +/- 1.66)% and (18.67 +/- 8.55)% respectively, and difference was significant between two groups (P < 0.01). There was negative correlation between PAS and semen volume, sperm density, percentage of forward motility, percentage of normal morphology (P < 0.01).
Conclusions:
There was very close relationship between sperm apoptosis and male infertility. FCM used to test sperm apoptosis is rapid, accurate, objective and reliable to analyse sperm functions and male fertility.
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