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[The effect of cryopreservation on human sperm chromosome]
1Department of Urology, First Hospital, Chongqing Medical University, Chongqing 400016, China.
Objectives:
To evaluate the effects of cryopreservation on human sperm chromosome.
Method:
Sperm chromosome were acquired using in vitro fertilization of zona-free hamster oocytes and human sperm. The frequency of sperm chromosome anomalies and sex chromosomes ratio before and after freezing and with different freezing methods were compared.
Results:
There were no significant differences of frequency of sperm chromosome anomalies among fresh, fast frozen and slow frozen sperm (9.40%, 7.48% and 8.74%) (P > 0.75) or ratios of sex chromosomes (P > 0.90).
Conclusions:
These studies indicate that cryopreservation does not exert effects on human sperm chromosome.
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