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[Effects of infrasound on ultrastructure of testis cell in mice]
Ya-Ning Wei1, Jing Liu, Qing Shu
1Department of Genetics and Developmental Biology, Xijing Hospital, Fourth Military Medical University, Xi'an, Shanxi 710033, China. weiyaning@sina.com.cn
Objectives:
To investigate the effects of infrasound on ultrastructure of testis in mouse.
Methods:
Twelve male BALB/C mice were randomly divided into three groups according to exposed duration on 1, 7 and 14 day. The mice were separately exposed to infrasound environment under 8 Hz/90 dB, 8 Hz/130 dB, 16 Hz/90 dB, 16 Hz/130 dB 2 hours per day. There was another control group which had three mice were separated into module with no infrasound. All the mice were killed on schedule. Then all the sections of testis were observed under electronic microscope. The alterations of structure and the chromatin were observed.
Results:
Some acute alteration in one day group was found in testis cell, such as cellular denaturation and necrosis, intercellular edema, mitochondria swelling, liposome hyperplasia. When the infrasound was up to 8 Hz/130 dB, the damage showed seriously. In 7 and 14 day group, the acute alteration was gradually decreased. A plenty of abnormal sperm were found. And other alteration was chromatin condense. The effect of variational frequency was important in ultrastructure.
Conclusions:
The infrasound markedly effected to testicular cell morphology and secreting function. Infrasound will lead to the alteration of procreation in mouse.