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Crystallization and preliminary X-ray crystallographic analysis of human Geminin coiled-coil domain
Michael Thépaut1, François Hoh, Christian Dumas
1Centre de Biochimie Structurale, CNRS UMR 5048 INSERM UMR 554, Faculté de Pharmacie, 15 Av Charles Flahault, 34060 Montpellier, France.
Abstract:
Initially discovered in Xenopus laevis, Geminin is a DNA replication initiation inhibitor found in higher eukaryotes. The coiled-coil domain of Human Geminin (termed GemH-37) has been crystallized by the vapor-diffusion sitting-drop method. A complete 1.74 A data set has been collected on a single orthorhombic crystal with unit cell parameters a = 25.25, b = 44.35, c = 68.58 A. Successful molecular replacement shows that GemH-37 is a dimeric parallel coiled-coil. Structural analysis is now in progress.
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