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[Application of Agilent 2100 Bioanalyzer in the study of differential gene expression]
Cui-Hua Liu1, Wen-Li Ma, Rong Shi
1Institute of Molecular Biology, First Military Medical University, Guangzhou 510515, China.
Objective:
To study the application of Agilent 2100 Bioanalyzer in the study of gene differential expression.
Methods:
The total RNAs were extracted and purified from Saccharomyces cerevisiae to synthesize double-stranded cDNAs by reverse transcription. Restriction display-PCR was employed to obtain the cDNA fragments, which were examined by Agilent 2100 Bioanalyzer and agarose gel electrophoresis.
Results:
The analysis showed that Agilent 2100 Bioanalyzer was more sensitive and faster to isolate and display the differentially expressed genes, providing at the same time accurate quantitative information for each fragment in the DNA sample.
Conclusion:
Agilent 2100 Bioanalyzer can be instrumental for the study of differential gene expression.