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Updated: Aug 9, 2026
Recombinant Retroviral Production and Infection of B Cells
Published on: February 19, 2011
[Construction of MHC I gene retroviral expression vector and its expression in hematopoietic cells]
Lan-Xiao Wu1, Kun-Yuan Guo, Jiang-Qi Li
1Department of Hematology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, China. wulx75@yahoo.com
Objective:
To construct the retroviral expression vector of BALB/c mouse H-2Dd gene and study its expression in C57BL/6 mouse hematopoietic cells (MHC).
Methods:
A retroviral vector pMSCV encoding H-2Dd gene was transduced into the packaging cell line PT67 by lipofectin, and the hematopoietic cells of C57BL/6(H-2Dd negative) were infected by the above viral supernatant. Reverse transcriptase-polymerase chain reaction and flow cytometry were employed to examine the expression of H-2Dd on the infected cells.
Results:
The cDNA encoding H-2Dd was correctly inserted into the vector pMSCV, as confirmed by restriction endonuclease digestion. The H-2Dd gene was integrated into the C57BL/6 mouse hematopoietic cell genome and expressed on the cell surface.
Conclusion:
Recombinant H-2Dd of BALB/c mouse retroviral expression vector has been successfully constructed and its expression obtained in C57BL/6 mouse hematopoietic cells, which may facilitate further study of the function of MHC in transplantation immunology.
