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Updated: Aug 15, 2026

Expansion of Human Peripheral Blood γδ T Cells using Zoledronate
Published on: September 9, 2011
[Interferon-gamma up-regulates Fas expression and increases Fas-mediated apoptosis in tumor cell lines]
Hang Zheng1, Rong-Cheng Luo, Luo-Sheng Zhang
1Department of Oncology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Objective:
To investigate effect of interferon-gamma (IFN-gamma) on Fas expression and Fas-mediated apoptosis in tumor cell lines.
Methods:
Fas expression was detected by flow cytometry, and the tumor cell apoptosis evaluated by enzyme-linked immunosorbent assay and AnnexinV staining assay.
Results:
All the 4 tumor cell lines used in this study, e.g. gastric cancer cell lines Kato-III AGS, lung cancer cell line A549 and laryngeal cancer cell line Hep-2, expressed Fas protein to varied degrees. Fas expression was up-regulated by IFN-gamma (P<0.05), which alone was enough to induce apoptosis in tumor cells and increase the susceptibility of them to anti-Fas antibodies. IFN-gamma induced Fas expression up-regulation and tumor cell apoptosis in a time-dependent manner.
Conclusion:
Fas-mediated apoptosis is one of the mechanisms for IFN-gamma to exercise its anti-tumor effect.
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