An integrated approach to lipid profiling: enzymatic determination of cholesterol and triglycerides with a...

We describe enzymatic methods for determination of cholesterol and triglycerides (triacylglycerols), adapted for use with a centrifugal analyzer. Triglycerides are determined by a two-point kinetic method, which utilizes a single glycerol standard for calibration and provides for a within-run reagent blank correction. The endpoint determination of cholesterol combines the unique blanking capabilities of the centrifugal analyzer to correct for sample and reagent blank interferences. Results correlate well with those obtained by procedures standardized by the Lipid Research Clinics Program for use with a continuous-flow system.