Recent advances in capillary isoelectric focusing: 1997-2001

Area of Science:

• Analytical Chemistry
• Biochemistry
• Separation Science

Background:

• Capillary isoelectric focusing (CIEF) is a powerful separation technique.
• Previous reviews have documented CIEF developments.
• Continued progress in CIEF technology is essential for various applications.

Purpose of the Study:

• To review methodological developments in CIEF from 1997-2001.
• To summarize applications and technological progress in CIEF.
• To highlight advancements in CIEF for proteomics and other fields.

Main Methods:

• Review of published literature on CIEF between 1997-2001.
• Analysis of experimental setups, sample preparation, and detection schemes.
• Discussion of two-dimensional separations and coupling with other techniques.

Main Results:

• Methodological advancements in CIEF, including capillary coatings, salt management, and additives.
• Progress in carrier ampholyte-free CIEF and computer simulations.
• Developments in microchannel IEF, advanced detection (imaging, fluorescence, chemiluminescence), and micropreparation.

Conclusions:

• Significant progress was made in CIEF technologies and applications during the review period.
• CIEF advancements are crucial for proteomics, noncovalent complex analysis, and microorganism separations.
• The review provides a comprehensive overview of CIEF developments for researchers in the field.