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[Expression of cell adhesion molecules in acute leukemia cell]
Xiaoping Ju1, Min Peng, Xiaoping Xu
1Department of Hematology, the Second Military Medical University, Shanghai 200433, China.
Objective:
To investigate the role of cell adhesion molecule in the development and extramedullary infiltration (EI) of acute leukemia.
Methods:
The expressions of neural cell adhesion molecule (NCAM) gene, intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule (VCAM-1) genes in 25 acute leukemia patients bone marrow cells were detected by microarray and reverse transcriptase-polymerase chain reaction (RT-PCR).
Results:
The expressions of NCAM, ICAM-1 and VCAM-1 gene were significantly higher in acute leukemia cells and leukemia cells with EI than in normal tissues and leukemia cells without EI, respectively, both by cDNA microarray and by RT-PCR.
Conclusion:
The cDNA microarray is a powerful technique in analysis of acute leukemia cells associated genes. High expressions of cell adhesion molecule genes might be correlated with leukemia pathogenesis and infiltration of acute leukemia cell.