Related Experiment Video
Updated: Aug 17, 2026

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
Published on: February 3, 2017
Sterol composition of mycelia of the plant pathogenic ascomycete Leptosphaeria maculans
K M Griffiths1, A Bacic, B J Howlett
1School of Botany, the University of Melbourne 3010, Victoria, Australia.
Abstract:
Analysis of sterols in mycelia of the ascomycete, Leptosphaeria maculans by gas chromatography-mass spectrometry revealed that ergosterol comprised 95% of the total sterols, with eight other sterols comprising the remaining 5%. Six of these latter sterols were putative precursors of ergosterol and their presence suggested a pathway for ergosterol biosynthesis in this fungus. Ergosterol biosynthesis in fungi is inhibited by the triazole antifungal agent flutriafol. When L. maculans was grown in the presence of flutriafol, ergosterol content decreased while two 14 alpha-methylated sterols, 24-methylene dihydrolanosterol and obtusifoliol, accumulated.
Related Concept Videos
Bacterial Phylum Planctomycetes
Bacterial Phylum Actinobacteria
Fungal Group Zygomycota
Fungal Phylum Microsporidia
Fungal Phylum Ascomycota
Fungal Phylum Basidiomycota

