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Related Experiment Videos

Alpha5beta1 integrin mediates strong tissue cohesion.

Elizabeth E Robinson1, Kathleen M Zazzali, Siobhan A Corbett

  • 1Department of Surgery, University of Medicine and Dentistry-Robert Wood Johnson Medical School, CAB Room 7070, New Brunswick, NJ 08648, USA.

Journal of Cell Science
|December 17, 2002
PubMed
Summary
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Integrins, traditionally cell-substratum adhesion molecules, can mediate strong cell-cell cohesion in 3D cultures. This integrin function relies on dimeric fibronectin and enhances intercellular cohesion in cellular aggregates.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Biophysics

Background:

  • Integrins and cadherins are key adhesion systems with distinct roles.
  • Integrins are primarily known for cell-extracellular matrix (ECM) interactions.
  • Cadherins mediate strong cell-cell adhesion, crucial for tissue integrity.

Purpose of the Study:

  • To investigate the potential of integrins to mediate intercellular cohesion in 3D cell cultures.
  • To challenge the traditional view of integrins as solely cell-substratum adhesion molecules.
  • To explore the mechanism by which integrins might influence cell-cell adhesion in a 3D environment.

Main Methods:

  • Transfection of Chinese hamster ovary (CHO-B2) cells to express alpha5beta1 integrin.
  • Formation and analysis of 3D cell aggregates.

Related Experiment Videos

  • Quantification of aggregate cohesivity using tissue surface tensiometry.
  • Assessment of fibronectin's role using fibronectin-null cells and varying fibronectin concentrations (dimeric vs. monomeric).
  • Inhibition studies using RGD peptides to confirm integrin specificity.

    • Main Results:

    • Cells expressing alpha5beta1 integrin formed compact, spherical 3D aggregates.
    • Alpha5beta1 integrin conferred strong cohesivity (sigma=8.22+/-0.68 dynes/cm) to cell aggregates.
    • This integrin-mediated cohesion was independent of cadherin expression and stronger than N-cadherin mediated cohesion.
    • Aggregate formation required dimeric fibronectin, with monomeric fibronectin being ineffective.
    • RGD peptide inhibition confirmed alpha5beta1 integrin specificity.

    Conclusions:

    • Integrins, specifically alpha5beta1, can mediate strong intercellular cohesion in 3D cellular aggregates.
    • This integrin function is dependent on dimeric fibronectin, acting as a bridge between adjacent cells.
    • These findings reveal a novel role for integrins in promoting cell-cell adhesion within a 3D matrix, distinct from their traditional ECM interactions.