Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

PX domain takes shape.

Frans B Wientjes1, Anthony W Segal

  • 1Department of Medicine, University College London, United Kingdom.

Current Opinion in Hematology
|December 17, 2002
PubMed
Summary

Phox homology (PX) domains bind phosphoinositides, directing proteins to cell membranes. These domains are crucial for cell signaling, protein sorting, and vesicle fusion, linking signaling networks to membrane protein assembly.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identifying high-impact variants and genes in exomes of Ashkenazi Jewish inflammatory bowel disease patients.

Nature communications·2023
Same author

Large-scale sequencing identifies multiple genes and rare variants associated with Crohn's disease susceptibility.

Nature genetics·2022
Same author

Genetic analysis of four consanguineous multiplex families with inflammatory bowel disease.

Gastroenterology report·2021
Same author

Correction: Insights into the genetic epidemiology of Crohn's and rare diseases in the Ashkenazi Jewish population.

PLoS genetics·2019
Same author

Variations in the Phagosomal Environment of Human Neutrophils and Mononuclear Phagocyte Subsets.

Frontiers in immunology·2019
Same author

Elevation in Cell Cycle and Protein Metabolism Gene Transcription in Inactive Colonic Tissue From Icelandic Patients With Ulcerative Colitis.

Inflammatory bowel diseases·2018

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Background:

  • Protein domains that bind phosphoinositides are key regulators of membrane targeting.
  • Phox homology (PX) domains are conserved protein modules involved in diverse cellular processes.

Purpose of the Study:

  • To highlight the role of PX domains in phosphoinositide binding and protein localization.
  • To describe the structural features and binding specificities of PX domains.

Main Methods:

  • Literature review of studies on PX domain function.
  • Analysis of conserved motifs and binding affinities.

Main Results:

  • PX domains bind various phosphoinositides, including PI(3)P, PI(3,4)P2, and PI(4,5)P2.
  • PX domains contain a PXXP motif for interaction with Src homology 3 (SH3) domains.
  • PX domains are implicated in cell signaling, protein sorting, vesicle fusion, and neutrophil superoxide generation.

Conclusions:

  • PX domains are versatile adaptors linking phosphoinositide signaling to protein localization at membranes.
  • PX domain interactions facilitate complex cellular functions, including signal transduction and membrane trafficking.

Related Experiment Videos