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Related Experiment Videos

Rapid bacterial counts in metal working fluids.

J L Sloyer1, T J Novitsky, S Nugent

  • 1Associates of Cape Cod, Inc., Falmouth, MA 02540, USA.

Journal of Industrial Microbiology & Biotechnology
|December 17, 2002
PubMed
Summary

A new automated fluorescent method provides rapid bacterial counts in metalworking fluids. This BactiFluor technique offers a quick and accurate alternative to traditional dip-slide cultures for fluid analysis.

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Area of Science:

  • Microbiology
  • Analytical Chemistry
  • Industrial Hygiene

Background:

  • Metalworking fluids (MWFs) are susceptible to bacterial contamination, impacting fluid performance and operator health.
  • Traditional methods for bacterial enumeration, such as dip-slide cultures, are time-consuming.
  • Rapid and accurate methods are needed for effective monitoring of microbial contamination in industrial fluids.

Purpose of the Study:

  • To evaluate a novel automated fluorescent method, BactiFluor, for rapid estimation of viable bacteria in metalworking fluids.
  • To compare the performance of the BactiFluor method against standard dip-slide cultures.

Main Methods:

  • A rapid (10-second) automated fluorescent assay was employed.
  • The BactiFluor method was used to quantify viable bacteria in 107 metalworking fluid samples.
  • Results were compared with those obtained from conventional dip-slide cultures.

Main Results:

  • The BactiFluor method demonstrated excellent agreement with dip-slide cultures for 107 metalworking fluid samples (correlation coefficient, r=0.99).
  • The method also showed favorable comparison with dip-slide cultures for an additional 30 metal processing fluids.
  • The automated fluorescent method provided results in just 10 seconds.

Conclusions:

  • The BactiFluor automated fluorescent method is a rapid and reliable tool for estimating viable bacteria in metalworking fluids.
  • This method offers a significant time advantage over traditional culture-based techniques.
  • The BactiFluor assay is suitable for routine monitoring of microbial contamination in various metal processing fluids.

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