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Updated: Sep 28, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus
Keiichi Goto1, Tomoko Omura, Yukihiko Hara
1Laboratory of QC and Food Analysis, Food Research Institute, Mitsui Norin Co., Ltd., Shizuoka 426-0133, Japan.
Abstract:
A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5' end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.
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