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Production and characterization of tannase from Bacillus cereus KBR9
Keshab C. Mondal1, Debdulal Banerjee, Rintu Banerjee
1Microbiology Laboratory, Department of Botany and Forestry, Vidyasagar University, Midnapore 721 102, West Bengal, India.
The Journal of General and Applied Microbiology
|December 17, 2002
Abstract:
A tannase-producing soil bacteria has been isolated and identified as Bacillus cereus. It can degrade tannic acid and produce maximum tannase (0.22 U/ml) at stationary phases of growth (24 h). Maximum growth and enzyme production occurred with initial medium pH of 4.5-5.0. Partial purified tannase showed optimum activity at pH 4.5 and 40 degrees C. It remains stable up to 30 degrees C and pH 4.5 to 5.0. The enzyme is salt tolerant, stable up to 2 m of NaCl and retains 82% original activity in 3 m.