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Updated: Sep 28, 2026
Multi-Locus Sequencing for Strain Typing of Toxoplasma gondii
Published on: May 26, 2026
Development of a rapid polymerase chain reaction-ELISA assay using polystyrene beads for the detection of Toxoplasma
E Martínez1, E Carmelo, R Alonso
1Department of Parasitology, Faculty of Pharmacy, niversity of La Laguna, La Laguna, Tenerife, Spain.
Aims:
To develop a rapid colourimetric assay for the detection of Toxoplasma gondii DNA using polystyrene beads as solid support.
Methods And Results:
A nested-polymerase chain reaction (PCR)-ELISA assay for the detection of T. gondii DNA was standardized by optimizing the hybridization time and probe concentration. Its detection threshold was then determined and compared with Southern blotting hybridization. These were found to be equivalent, but the PCR-ELISA-beads test is easier to perform and the turnaround time is much shorter than with Southern blot.
Conclusions:
The PCR-ELISA-beads assay is a valuable tool for the detection of T. gondii DNA.
Significance And The Impact Of The Study:
Our results demonstrate that this PCR-ELISA assay, using polystyrene beads, can be used as a routine diagnostic test for the detection of T. gondii in clinical laboratories.
