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Updated: Aug 19, 2026

Spectral Confocal Imaging of Fluorescently tagged Nicotinic Receptors in Knock-in Mice with Chronic Nicotine Administration
Published on: February 10, 2012
Immobilized nicotinic receptor stationary phases: going with the flow in high-throughput screening and
Ruin Moaddel1, Irving W Wainer
1Gerontology Research Center, National Institute on Aging, National Institutes of Health, 5600 Nathan Shock Drive, Baltimore, MD 21224-6825, USA.
Abstract:
The nicotinic acetylcholine receptor (nAChR) subtypes alpha3beta4-nAChR and alpha4beta2-nAChR have been immobilized and the resulting stationary phases used to determine binding affinities. The alpha3beta4-nAChR column was coupled to a C(18) column and a mixture of 18 compounds was sorted into ligands and non-ligands for the alpha3beta4-nAChR. The results demonstrate that the nAChR stationary phases can be used for on-line high-throughput screening (HTS).
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