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Association of Bcl-2 with cyclin a/Cdk-2 complex and its effects on Cdk-2 activity
Elvira Crescenzi1, Maria Sannino, Gilda Tonziello
1Dipartimento di Biologia e Patologia Cellulare e Molecolare, University of Naples Federico II, Via S. Pansini, 5, 80131 Naples, Italy, and ASI, Rome, Italy. elviracescenzi@hotmail.com
Abstract:
In the human endometrial carcinoma cell line HEC1B, the overexpression of Bcl-2 leads to a G2/M cell cycle arrest. The experiments presented in this work suggest a direct interaction between the antiapoptotic protein Bcl-2 and the cyclin-dependent kinase Cdk-2 and suggest that such interaction is cell cycle dependent. While further experiments are currently under way in our laboratory to elucidate the significance of Cdk-2/Bcl-2 complexes in PCD and cell cycle regulation, we demonstrate also a specific inhibitory function of Bcl-2 on the activity of the coprecipitated kinase.