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Updated: Sep 28, 2026
Screening Assays to Characterize Novel Endothelial Regulators Involved in the Inflammatory Response
Published on: September 15, 2017
Analysis of in vivo endothelial cell activation applying RT-PCR following endothelial cell isolation by laser
S A Asgeirsdottir1, N Werner, G Harms
1Department of Pharmacokinetics and Drug Delivery, Medical Biology Section, Groningen University Institute for Drug Exploration (GUIDE), Groningen, the Netherlands. s.asgeirsdottir@farm.rug.nl
Abstract:
Laser dissection microscopy was applied to isolate endothelial cells from tumors obtained from mice treated with TNF-alpha. RNA integrity was demonstrated from whole sections and dissected cells after acetone fixation and hematoxylin staining. RT-PCR for GAPDH, CD31, VCAM-1, ICAM-1, and E-selectin was successfully performed on these samples. These data demonstrate the feasibility of analyzing local endothelial cell responses in diseased tissues at the level of gene expression.
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