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Matrix vesicles in aging cartilage.

E Bonucci, L C Dearden

    Federation Proceedings
    |February 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Aging cartilage calcification is regulated by matrix vesicles. Impaired inorganic substance spread in aged cartilage suggests matrix vesicles play a key role in calcium binding and collagen formation.

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    Area of Science:

    • Biochemistry
    • Cell Biology
    • Histology

    Background:

    • Calcification is a complex biological process.
    • Matrix vesicles are known to play a role in initiating calcification in various tissues.
    • Aging can affect the structural integrity and biochemical properties of cartilage.

    Purpose of the Study:

    • To investigate the role of matrix vesicles in cartilage calcification during aging.
    • To understand the mechanisms underlying impaired calcification in aged cartilage.
    • To explore the relationship between matrix vesicles, collagen formation, and calcium binding in aging cartilage.

    Main Methods:

    • Electron microscopy was used to examine costal and tracheal cartilage from rats and human costal cartilage.
    • Analysis focused on the early stages of calcification and the structure of matrix vesicles.

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  • Chondrocyte degeneration and pericellular halo formation were also observed.

    • Main Results:

    • Matrix vesicles induce and regulate early calcification in aged cartilage, similar to other tissues.
    • Inorganic substance spread from vesicles into the matrix is often impaired in aged cartilage due to low calcium ion concentration or unsuitable matrix structure.
    • Matrix vesicles exhibit a higher affinity and calcium-binding potential than other cartilage matrix components.
    • Chondrocyte degeneration ('Verdämmerung der Zellen') leads to matrix vesicle formation and pericellular halos.
    • Disrupted matrix vesicles contribute to pericellular aggregates and can induce collagen fibril formation.

    Conclusions:

    • Matrix vesicles are crucial for initiating and regulating cartilage calcification, even in aging.
    • Impaired calcification in aged cartilage is linked to reduced calcium ion availability and matrix structural changes.
    • Matrix vesicles possess significant calcium-binding properties that are maintained even after their content is released into the matrix.
    • The release of matrix vesicle contents may contribute to collagen formation and influence the calcification process in aging cartilage.