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Functional maturation during bovine granulopoiesis.
V Van Merris1, E Meyer, C Burvenich
1Ghent University, Faculty of Veterinary Medicine Department of Physiology, Biochemistry and Biometrics, Salisburylaan 133 9820 Merelbeke, Belgium.
Journal of Dairy Science
|December 19, 2002
Summary
Bovine granulopoiesis involves sequential functional development. Immature cells express receptors, while mature cells gain phagocytic and respiratory burst activities, indicating distinct maturation stages.
Area of Science:
- Immunology
- Cell Biology
- Veterinary Science
Background:
- Granulopoiesis involves significant cellular changes in bone marrow.
- These changes lead to specialized proteins for polymorphonuclear leukocyte functions.
- Understanding these events is crucial for bovine immune function research.
Purpose of the Study:
- To sequence the functional development of granulocytic bone marrow cells in cattle.
- To analyze the expression of key receptors and functions during maturation.
- To establish a timeline for bovine granulopoiesis.
Main Methods:
- Bone marrow cells were collected from healthy cows.
- Cells were separated into maturation stages via density gradient centrifugation.
- Fc-IgG2 receptor, CD11b expression, phagocytosis, respiratory burst, and myeloperoxidase activity were assessed.
Main Results:
- Immature cells expressed Fc-IgG2 receptor and CD11b.
- Phagocytic ability developed in the myelocytic stage, with mature cells being most capable.
- Respiratory burst activity was limited to band and segmented cells; myeloperoxidase was present throughout.
Conclusions:
- Bovine granulopoiesis exhibits a sequential expression of functions and receptors.
- Maturation stages are characterized by specific functional capabilities.
- A model of sequential biochemical maturation during bovine granulopoiesis is proposed.