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Updated: Sep 28, 2026
FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
[Preimplantation genetic diagnosis]
Xiangge Meng1, Lihong Zhang, Yunling Dong
1Reproductive Medical Research Center, Shandong Institute for Family Planning, Jinan 250002, China.
Objective:
To evaluate the clinical effectiveness of preimplantation genetic diagnosis (PGD) for the couples having a higher risk of genetic diseases with fluorescence in situ hybridization (FISH).
Methods:
Ovarian hyperstimulation, oocyte retrieval and in vitro fertilization were performed in 4 cases. Blastomere biopsy were performed from the embryos at the 6 - 8 cell stage. Genetic diagnosis was identified by single blastomere FISH. The female embryos or normal embryos were transferred into uterus.
Results:
Twelve embryos were available for embryo biopsy. Twenty cells were collected. Seventeen nuclei were found after fixation. Hybridization signals of 11 embryos were clear and 8 embryos were transferred into uterus. One clinical pregnancy was achieved, and 1 normal female infant was delivered on september 14, 2001.
Conclusion:
PGD is very valuable for avoiding selective abortion and birth of affected child of the couples having a higher risk of genetic diseases by detecting embryo sex and chromosome number using FISH.
