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Related Experiment Videos

Anion selectivity by the sodium iodide symporter.

J Van Sande1, C Massart, R Beauwens

  • 1Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire, University of Brussels, School of Medicine, Erasmus Hospital, B 1070 Brussels, Belgium. jvsande@ulb.ac.be

Endocrinology
|December 19, 2002
PubMed
Summary

The human sodium-iodide symporter (NIS) protein directly transports iodide and other anions, mirroring thyroid cell transporter properties. This clarifies NIS function in thyroid iodide uptake, crucial for thyroid hormone synthesis.

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Area of Science:

  • Molecular Biology
  • Endocrinology
  • Cell Physiology

Background:

  • The thyroid iodide transporter (NIS) is essential for thyroid hormone synthesis.
  • Previous studies using electrophysiology in Xenopus oocytes yielded conflicting anion selectivity data for NIS compared to thyroid cells.

Purpose of the Study:

  • To directly compare the transport properties of human NIS expressed in COS-7 cells with those in FRTL5 thyroid cells.
  • To elucidate the anion selectivity and substrate affinity of the human NIS transporter.

Main Methods:

  • Stable transfection of human NIS into COS-7 cells.
  • Direct measurement of radioisotope uptake: (125)I(-), (186)ReO(4)(-), and (99m)TcO(4)(-).
  • Assessment of inhibition by competing anions: ClO(4)(-), SCN(-), ClO(3)(-), ReO(4)(-), and Br(-).

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Main Results:

  • Human NIS stably expressed in COS-7 cells exhibited transport properties consistent with those found in FRTL5 thyroid cells.
  • The determined order of anion affinity for NIS was: ClO(4)(-) > ReO(4)(-) > I(-) >/= SCN(-) > ClO(3)(-) > Br(-).
  • Dysidenin was confirmed as an inhibitor of NIS, consistent with observations in dog thyroid tissue.

Conclusions:

  • The intrinsic properties of the human sodium-iodide symporter (NIS) fully account for the previously observed characteristics of the thyroid iodide transporter.
  • This study provides direct evidence for the anion transport characteristics of human NIS, resolving discrepancies from prior electrophysiological studies.