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Spontaneous rosette formation by mouse L cells.

M Papamichail, C Gutierrez, A Temple

    Immunology
    |January 1, 1976
    PubMed
    Summary
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    Mouse fibroblasts spontaneously form rosettes with red blood cells, differing from T cell mechanisms. This cell-cell interaction is temperature-dependent and sensitive to specific chemical treatments.

    Area of Science:

    • Cell Biology
    • Immunology

    Background:

    • Mouse fibroblasts exhibit spontaneous rosette formation with erythrocytes.
    • This interaction differs from complement (C3) or Fc receptor-mediated rosettes.

    Purpose of the Study:

    • To investigate the characteristics and mechanism of spontaneous rosette formation by mouse fibroblasts.
    • To compare this mechanism with that of T cell rosette formation.

    Main Methods:

    • Studied rosette formation kinetics, temperature dependence, and sensitivity to various chemical and physical treatments.
    • Tested inhibition by antisera, fixation, cell killing agents, and drugs like vinblastine and cytochalasin B.

    Main Results:

    • Spontaneous rosette formation is optimal at 37°C, rapid (30 min), and inhibited by fixation, cell killing, and vinblastine.

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  • Rosetting is not inhibited by antisera to fibroblast membrane components, cytochalasin B, or by low temperatures.
  • Fibroblasts do not form C3 or Fc rosettes or bind aggregated gammaglobulin.

    • Conclusions:

    • The mechanism of fibroblast spontaneous rosette formation differs from that of T cells.
    • Kinetics, temperature sensitivity, and drug responses suggest a unique pathway for fibroblast-erythrocyte interaction.