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Related Experiment Videos

FGF10 maintains stem cell population during mouse incisor development.

Hidemitsu Harada1, Takashi Toyono, Kuniaki Toyoshima

  • 1Second Department of Oral Anatomy and Cell Biology, Kyushu Dental College, 2-6-1, Manazuru, Kokurakita-ku, Kitakyushu, 803-8580, Japan. hide-h@mail.kyu-dent.ac.jp

Connective Tissue Research
|December 20, 2002
PubMed
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Fibroblast growth factor 10 (FGF10) is crucial for maintaining the stem-cell compartment in developing mouse incisors. FGF10 deficiency prevents cervical loop formation, highlighting its essential role in tooth germ development.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Dental Research

Background:

  • Mouse incisors possess a unique cervical loop structure responsible for dental epithelium formation.
  • The stem-cell compartment's development in incisors is critical for continuous tooth growth.
  • Fibroblast growth factors (FGFs) are known regulators of embryonic development.

Purpose of the Study:

  • To investigate the role of fibroblast growth factor 10 (FGF10) and FGF3 in the formation of the stem-cell compartment in developing mouse incisors.
  • To elucidate the specific function of FGF10 in maintaining the cervical loop structure.

Main Methods:

  • Analysis of FGF10 and FGF3 expression patterns during mouse incisor development (E14, post-E16).
  • Examination of incisor development in FGF10-deficient (null) mice.

Related Experiment Videos

  • In vitro studies using neutralizing anti-FGF10 antibodies and recombinant FGF10 on incisor explants.

    • Main Results:

    • FGF10 and FGF3 were coexpressed in the dental papilla at E14.
    • Post-E16, FGF10 was expressed in mesenchymal cells underlying the cervical loop, while FGF3 was not.
    • FGF10-null mice exhibited normal early germ development but failed to form the cervical loop later.
    • Anti-FGF10 antibodies induced apoptosis in the cervical loop, which was rescued by recombinant FGF10.

    Conclusions:

    • FGF10 is essential for the formation and maintenance of the cervical loop in developing mouse incisors.
    • FGF10 plays a critical role in preserving the stem-cell compartment within the incisor tooth germ.
    • These findings identify FGF10 as a key molecular factor in regulating incisor stem cell biology.