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The conservation and regulation of rat DSP-PP gene
H H Ritchie1, X R Li, C T Hanks
1Department of Cariology, Restorative Sciences, and Endodontics, University of Michigan School of Dentistry, 1011 North University, Ann Arbor, MI 48109-1078, USA. helenar@umich.edu
Abstract:
Two highly expressed noncollagenous proteins associated with dentin mineralization, dentin sialoprotein (DSP) and phosphophoryn (PP), are encoded by a single DSP-PP transcript. To better understand how DSP-PP transcripts are regulated, we have determined the DSP-PP transcription start site, sequenced its 5' flanking region, and analyzed the transcriptional activity of the gene promoter out to -1615 bp. Comparison of the rat cDNA sequence with the mouse, rat and human genes clearly indicates high sequence conservation within the DSP-PP 5' flanking region, implicating the possible presence of highly conserved gene regulatory cis elements. Among a number of conserved transcription sites identified in the 5' flanking region, we demonstrate that the conserved Y box sequence (ATTGG) can specifically bind nuclear extracts from mouse MDPC23 cells. This sequence (located within the -57 bp/-52 bp 5' flanking region) therefore likely represents one DSP-PP transcriptional regulatory sequence.
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