Sex steroids regulate intermediary metabolism in Oreochromis mossambicus

The effect of long-term administration of testosterone, progesterone, and a synthetic estrogen, diethylstilbestrol (DES), on intermediary metabolism was studied in a freshwater fish Oreochromis mossambicus. The present study reveals that testosterone, progesterone, and Des specifically control key enzymes involved in carbohydrate, protein and lipid metabolism in the liver of O. mossambicus implying a general influence of sex steroids on intermediary metabolism. The activities of malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH), isocitrate dehydrogenase (ICDH), glucose 6 phosphatase (G-6-Pase), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) are either stimulated or inhibited following the administration of sex steroids. The long-term in vivo i.p. injection of sex steroids intensely reveals that testosterone and progesterone are hyperglycemic, DES is hypoglycemic, testosterone and DES lipogenic, and progesterone antilipogenic (lipolytic) in the present study. It is also established that amino acid catabolism, mostly that of alanine, may be a major source of substrate for gluconeogenesis. A genomic mode of action is proposed for sex steroids for long term treatment, as their action is sensitive to transcription and translation inhibitors.