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Synthesis and characterization of pi-stacked phenothiazine-labeled oligodeoxynucleotides
S A Nadeem Hashmi1, Xi Hu, Chad E Immoos
1Department of Chemistry, Paul M. Gross Chemical Laboratory, Duke University, Durham, North Carolina 27708, USA.
Organic Letters
|December 20, 2002
Abstract:
[reaction: see text] A facile procedure for the incorporation of N-methyl phenothiazine as the terminal nucleoside in oligodeoxynucleotides is reported. The phenothiazine nucleoside analogue is synthesized and then incorporated into DNA using an automated DNA solid-phase synthesizer. Phenothiazine-labeled oligodeoxynucleotides form stable B-form duplexes with higher melting temperatures compared to unlabeled DNA duplexes.