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Updated: Jul 15, 2026

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A simplified method for the detection of Y chromosome microdeletions in infertile men using a multiplex
Christine Charpenel1, Anne-Sophie Guillon, Olivier Dorson
1Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R Poincaré, 92380 Garches, France.
Abstract:
Sixteen sequence-tagged sites (STSs) were combined in five amplification reactions, to screen for deletions of DNA fragments located within the AZFa, AZFb, and AZFc regions of the Y chromosome. This multiplex strategy is fast and reliable, and most of the azoospermia-associated deletions reported so far are detected with this simplified method. Internal control STSs are included that allow discrimination between deletion and failure of amplification.
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