Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Opioid receptor-induced GTPgamma35S binding during mouse development.

Joshua F Nitsche1, John E Pintar

  • 1Department of Neuroscience and Cell Biology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, New Jersey 08854, USA.

Developmental Biology
|December 20, 2002
PubMed
Summary

Functional activation of mu-opioid receptors (MOR) and delta-opioid receptors (DOR) in developing mice shows distinct temporal and regional patterns. This suggests developmental regulation of opioid receptor signaling pathways.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Construction and Evaluation of a Ballistic Gelatin-Based Simulator for Transcervical Chorionic Villus Sampling.

Journal of ultrasound in medicine : official journal of the American Institute of Ultrasound in Medicine·2025
Same author

Meningeal regulatory T cells inhibit nociception in female mice.

Science (New York, N.Y.)·2025
Same author

Oxa-Iboga alkaloids lack cardiac risk and disrupt opioid use in animal models.

Nature communications·2024
Same author

Regulatory T cell-derived enkephalin gates nociception.

bioRxiv : the preprint server for biology·2024
Same author

Low-Cost Task Trainer for In Utero Fetal Stent Placement.

Simulation in healthcare : journal of the Society for Simulation in Healthcare·2023
Same author

The effects of heparin, aspirin, and maternal clinical factors on the rate of nonreportable cell-free DNA results: a retrospective cohort study.

American journal of obstetrics & gynecology MFM·2022

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Pharmacology

Background:

  • G-protein-coupled receptors (GPCRs) are crucial for numerous physiological functions.
  • Limited understanding exists regarding the developmental activation patterns of GPCRs, particularly opioid receptors.
  • Investigating opioid receptor functional activation during ontogeny is essential for understanding neurodevelopment.

Purpose of the Study:

  • To examine the functional activation of mu-opioid receptors (MOR) and delta-opioid receptors (DOR) during mouse embryonic and fetal development.
  • To map the spatiotemporal emergence of MOR and DOR functional activity in the developing brain.
  • To compare the developmental timing of MOR and DOR functional activation with their gene expression.

Main Methods:

  • Utilized GTPgamma(35)S binding autoradiography on mouse embryo and fetus sections from embryonic day 11.5 (e11.5) to birth.

Related Experiment Videos

  • Assessed G-protein coupling in response to MOR-specific agonist DAMGO and DOR-specific agonist DPDPE.
  • Correlated functional activity with previously reported MOR-1 and DOR-1 mRNA expression data.

    • Main Results:

    • MOR functional activation was first detected at e12.5 in the caudate-putamen (CPU), expanding to other brain regions by e15.5.
    • DOR activity emerged later, first observed at e17.5 in the hypothalamus, pons, and medulla, and at postnatal day 1 (p1) in the CPU at lower levels than MOR.
    • While generally coinciding with MOR-1 and DOR-1 mRNA appearance, delayed functional activation was noted in trigeminal ganglia despite early MOR-1 expression.

    Conclusions:

    • Demonstrates distinct temporal and regional differences in the functional activation of MOR and DOR during development.
    • Highlights that the appearance of receptor mRNA does not always directly correlate with functional receptor activity.
    • Suggests that the intricate interactions between opioid receptors, G-proteins, and signaling cofactors are under developmental regulation.